目的对4周龄Fmr1基因敲除小鼠的耳蜗的GAD表达进行观察,探讨耳蜗GAD的表达是否受FMRP的影响。方法使用PCR技术对Fmr1基因敲除小鼠鉴定后对4周龄的Fmr1基因敲除小鼠和野生型小鼠各15只进行耳蜗的苏木精-伊红(HE)染色和GAD免疫组织化学的表达观察,数据采用多因素方差分析处理。结果耳蜗HE染色结果:4周龄组KO鼠较WT鼠形态学观察无差异。4周龄KO小鼠的耳蜗中GAD表达的平均阳性细胞数均高于WT小鼠,P<0.01,差异具有统计学意义。结论 GAD表达的改变可能与FMR1基因KO小鼠听源性惊厥发病有关。 Objective To observe the expression of GAD in the cochlea of ​​4-week-old Fmr1 knockout mice to investigate whether the expression of GAD in the cochlea is affected by FMRP. Methods Fifty-four Fmr1 knockout mice and 15 wild type mice were identified by FQ-1 knockout mice using PCR. The cochlear hematoxylin-eosin (HE) staining and GAD immunohistochemistry The observation of the data, the data using multiple factor analysis of variance. Results The results of cochlear HE staining: there was no difference in morphological observation between 4-week-old KO rats and WT rats. The average number of GAD positive cells in 4 weeks old KO mice was higher than WT mice (P <0.01), and the difference was statistically significant. Conclusion The change of GAD expression may be related to the onset of auditory seizure in FMR1 gene KO mice.