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目的探讨血红素加氧酶-1(heme oxygenase-1,HO-1)在高氧暴露早产鼠肺组织中的表达及银杏叶制剂(舒血宁)对HO-1 mRNA及HO-1蛋白表达的影响,为舒血宁防治早产儿高氧肺损伤提供理论依据。方法3日龄早产鼠随机分为高氧组、空气组和高氧+舒血宁组3组,于实验3 d及7 d时,分别用半定量反转录-聚合酶链反应法和免疫组织化学法检测各组早产鼠肺组织HO-1 mRNA的表达水平和HO-1蛋白质表达水平。结果实验第3天,空气组HO-1 mRNA、HO-1蛋白均有微弱表达;与空气组相比,高氧组HO-1 mRNA表达明显高于空气组(P<0.01),HO-1蛋白仅在巨噬细胞表达弱阳性(P<0.05);与高氧组相比,舒血宁+高氧组HO-1 mRNA的表达明显下降(P<0.01),但仍然高于空气组(P<0.05),HO-1蛋白弱阳性表达(P>0.05)。实验第7天,各组HO-1 mRNA均未见表达。空气组HO-1蛋白极微弱或无表达,高氧组HO-1蛋白在巨噬细胞呈强阳性表达,明显高于空气组(P<0.01);舒血宁+高氧组HO-1蛋白的表达较高氧组明显下降(P<0.01),但仍然高于空气组(P<0.01)。结论HO-1可能参与了早产鼠高氧肺损伤,舒血宁能够下调高氧暴露早产鼠肺组织中HO-1的表达水平。
Objective To investigate the expression of heme oxygenase-1 (HO-1) in the lungs of hyperoxia-exposed preterm rats and the expression of HO-1 mRNA and HO-1 protein in Ginkgo biloba extract (shu xi ning). The influence of Shuxuening on prevention and treatment of hyperoxia-induced lung injury in preterm infants was provided. Methods Three-day-old preterm rats were randomly divided into hyperoxia group, air group, and hyperoxia+shuxuening group. At the 3rd and 7th day of experiment, semi-quantitative reverse transcription-polymerase chain reaction and immunization were performed respectively. Histochemistry was used to detect the expression of HO-1 mRNA and the expression of HO-1 protein in lung tissue of each group of premature rats. Results On the third day of the experiment, HO-1 mRNA and HO-1 protein were weakly expressed in the air group. Compared with the air group, the HO-1 mRNA expression in the hyperoxia group was significantly higher than that in the air group (P<0.01). HO-1 Protein expression was only weakly positive in macrophages (P<0.05); compared with hyperoxia group, HO-1 mRNA expression in Shuxuening + hyperoxia group was significantly decreased (P<0.01), but still higher than that in air group ( P<0.05), weakly positive expression of HO-1 protein (P>0.05). On the 7th day of the experiment, no expression of HO-1 mRNA was observed in all groups. The HO-1 protein in the air group was very weak or not expressed. The HO-1 protein in the hyperoxia group was strongly positive in the macrophage, which was significantly higher than that in the air group (P<0.01); HO-1 protein in the Shuxuening + hyperoxia group. The expression of oxygen in the hyperoxia group was significantly decreased (P<0.01), but it was still higher than that in the air group (P<0.01). Conclusion HO-1 may be involved in hyperoxia-induced lung injury in premature rats. Shuxuening can down-regulate the expression of HO-1 in lung tissue of hyperoxia-exposed preterm rats.