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目的研究食管癌患者肿瘤组织中血管紧张素转换酶(ACE)基因多态性,探讨该基因与食管癌之间的关系。方法共检测50例食管癌患者组织标本,利用聚合酶链反应(PCR)检测ACE基因,观察插入型(ID型)、缺失型(DD型)和Ⅱ型3种基因型多态性分布,并计算I/D等位基因的比例,以10例癌旁正常组织为对照。结果食管癌患者肿瘤组织中ACE基因插入/缺失(I/D)多态性分布特点:ID型、DD型和Ⅱ型基因比例分别为16%,64%,20%,而I/D等位基因分布频率分别为20%和80%,对照组ID型、DD型和Ⅱ型3种基因型的比例分别为40%、30%、30%,其I/D等位基因分布频率分别为30%和70%,两组间DD型基因分布频率有统计学显著性差异(P<0.05)、I/D等位基因分布频率无显著性差异(P>0.05)。结论食管癌患者肿瘤组织中存在ACE基因I/D多态性,DD型基因分布较癌旁正常组织显著升高,结果表明DD基因型高表达可能与食管癌发病相关。
Objective To investigate the gene polymorphism of angiotensin-converting enzyme (ACE) in esophageal cancer tissues and to explore the relationship between the gene and esophageal cancer. Methods A total of 50 cases of esophageal cancer tissue samples were detected. The ACE gene was detected by polymerase chain reaction (PCR). The polymorphism distributions of insert type (ID), deletion type (DD) and type Ⅱ were observed. Calculate the proportion of I / D alleles to 10 cases of adjacent normal tissue as a control. Results The distribution of ACE gene insertion / deletion (I / D) polymorphism in esophageal cancer tissues was 16%, 64% and 20% respectively, while the I / D alleles The frequency of gene distribution was 20% and 80% respectively. The ID, DD and Ⅱ genotypes in control group were 40%, 30% and 30% respectively, and the frequencies of I / D alleles were 30 % And 70% respectively. There was a statistically significant difference in the distribution frequency of DD genotypes between the two groups (P <0.05). There was no significant difference in the distribution frequency of I / D alleles (P> 0.05). Conclusion ACE gene I / D polymorphism exists in esophageal cancer tissue, and the distribution of DD gene is significantly higher than that in adjacent normal tissues. The results show that the high expression of DD genotype may be associated with the pathogenesis of esophageal cancer.