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目的 研究促甲状腺激素释放激素 (thyrotrophin- releasing hormone,TRH)及其受体 (TRH receptor,TRH- R)在大鼠睾丸组织中的表达规律和在生殖发育调节中的作用 .方法 以大鼠睾丸组织总 RNA为模板 ,依据大鼠下丘脑中的前 TRH和垂体中的 TRH- R c DNA设计引物 ,进行反转录聚合
Objective To study the expression of thyrotrophin-releasing hormone (TRH) and its receptor (TRH receptor) in rat testis and its role in the regulation of reproductive development.Methods Rat testis Tissue total RNA as a template, based on the hypothalamus in rat pre-TRH and pituitary TRH-R c DNA primers designed for reverse transcription polymerization