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目的初步了解不同年龄人群可能感染克罗诺杆菌的情况,并比较从粪便样本中分离培养和鉴定克罗诺杆菌的方法。方法采用直接涂板和增菌后涂板的两种分离培养方法,生化实验和荧光定量PCR进行进一步鉴定,分种则用基因fusA序列分析的方法实现。结果本研究在1 285份不同年龄人群的粪便样本中分离得到克罗诺杆菌27株,阳性率为2.10%。各年龄组的阳性率,经过χ2检验差异无统计学意义。27株克罗诺杆菌分为4个种,主要为阪崎克罗诺杆菌和丙二酸盐克罗诺杆菌。用CSB增菌液和CCI选择性培养基要比用mLST/Van增菌液和EIA选择性培养基敏感性高(P<0.05)。结论克罗诺杆菌对不同年龄组的人群都有潜在的危害性,但主要为婴幼儿,因此要加强监测,提高防范意识。
Objective To understand the possible infection of Cronobacterium in different age groups and to compare the isolation and culture of Cronobacterium from stool samples. Methods Two kinds of isolation and culture methods, biochemical experiments and fluorescence quantitative PCR were used to further identify the direct-coated plates and the amplified plates, and the method of gene fusA sequence analysis was used for the classification. Results In this study, 27 strains of Cronobacterium were isolated from the stool samples of 1,285 different age groups, the positive rate was 2.10%. The positive rate of each age group, after χ2 test difference was not statistically significant. 27 strains of Corynebacterium were divided into 4 species, mainly Kesen Kakusaki and Malonate Bacillus cereus. The CSB-enriched and CCI-selective media were more sensitive than the mLST / Van broth and EIA-selective medium (P <0.05). Conclusions Cronobacter is potentially harmful to people of different age groups, but mainly for infants and young children. Therefore, monitoring and prevention awareness should be strengthened.