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目的:评价猕猴桃根提取物的体外、内抗肝癌作用。方法:猕猴桃根活性成分的分离、纯化采用传统的天然产物化学方法;不同组分对体外培养的肝癌Bel-7402细胞作用采用磺酰罗丹明B方法;猕猴桃根的体内抗肿瘤作用采用小鼠肿瘤和人肿瘤裸小鼠移植瘤模型评价。结果:猕猴桃根先用甲醇回流提取,然后用乙酸乙酯、氯仿、正丁醇萃取。氯仿提取物对肿瘤细胞增殖抑制作用最强,甲醇提取物次之。体内实验证实氯仿提取物有效地抑制小鼠肝癌模型和人肝癌裸小鼠移植瘤模型的生长,抑制率大概在38.0%。结论:猕猴桃根提取物有一定的抗肿瘤作用;其活性成分主要存在于极性较小的组分。
Objective: To evaluate the in vitro and in vivo anti-hepatocellular carcinoma effects of kiwifruit root extract. Methods: The separation and purification of kiwifruit root active ingredients were performed by traditional natural chemical methods. The effects of different components on the growth of Bel-7402 hepatocellular carcinoma cells in vitro were evaluated by sulfonyl rhodamine B method. And human tumor nude mice xenograft model evaluation. Results: Kiwifruit root was first extracted with methanol and then extracted with ethyl acetate, chloroform and n-butanol. Chloroform extract had the strongest inhibitory effect on tumor cell proliferation, followed by methanol extract. In vivo experiments confirmed that chloroform extract effectively inhibited the growth of mouse liver cancer model and human liver cancer xenograft model of nude mice, the inhibition rate was about 38.0%. Conclusion: The kiwifruit root extract has certain anti-tumor effect; its active ingredient mainly exists in the less polar components.