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目的观察血清白介素-18(IL~(-1)8)水平及胎膜中基质金属蛋白酶-2(MMP-2)表达与足月前胎膜早破(PPROM)发生的关系。方法选取PPROM孕妇(PPROM组)及胎膜未破孕妇(对照组)各60例,根据产后病检结果将入选孕妇分为炎症组35例及非炎症组85例,采集各组患者肘静脉血,采用夹心酶联免疫吸附法(ELISA法)检测两组孕妇血清中IL-8水平,并于产后采集入选对象胎膜破口处胎膜组织,免疫组化染色法镜下观察检测细胞浆中MMP-2表达情况。结果 PPROM组血清IL~(-1)8水平、胎膜组织中MMP-2表达均高于对照组(P均<0.05);炎症组产妇血清IL~(-1)8水平、胎膜组织中MMP-2高于非炎组(P均<0.05)。结论 PPROM与孕期感染有关,IL~(-1)8及MMP-2表达异常参与PPROM发病。
Objective To investigate the relationship between serum interleukin-18 (IL-8) 8 and matrix metalloproteinase-2 (MMP-2) expression in preterm premature rupture of membranes (PPROM). Methods The PPROM pregnant women (PPROM group) and the unperforated pregnant women (control group), 60 cases each, were divided into 35 cases of inflammatory group and 85 cases of non-inflammatory group according to the postpartum pathological examination results. The elbow venous blood . The levels of IL-8 in serum of the two groups were detected by sandwich enzyme-linked immunosorbent assay (ELISA), and the fetal membranes in the fetal membranes were collected after delivery. Immunohistochemical staining was used to detect the cytoplasm MMP-2 expression. Results The level of IL - 8 in PPROM group was significantly higher than that in control group (P <0.05). The level of IL - 8 in serum of PPROM group, MMP-2 was higher than non-inflammation group (P <0.05). Conclusions PPROM is related to infection during pregnancy. The abnormal expression of IL-8 and MMP-2 may play an important role in the pathogenesis of PPROM.