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目的:建立两头尖的高效液相色谱(HPLC)指纹图谱。方法:采用HPLC法。色谱柱为Phemomenex Gemini C18,流动相为0.1%磷酸-乙腈(梯度洗脱),流速为1 ml/min,检测波长为206 nm,柱温为30℃,进样量为20μl。以竹节香附素A为参照物,分析13批两头尖样品,采用“中药色谱指纹图谱相似度评价系统”软件进行相似度分析,并采用SPSS 13.0统计软件对样品进行聚类分析。结果:13批两头尖样品有11个共有峰,相似度>0.90。经验证,两头尖的指纹图谱与对照指纹图谱具有较好的一致性。13批药材产地中吉林桦甸、吉林蛟河、吉林天岗、吉林左家、黑龙江尚志、吉林舒兰、吉林通化、吉林抚松为第1类,黑龙江哈尔滨、黑龙江亚布力镇、辽宁清原、黑龙江一面坡、山东济南为第2类。结论:所建指纹图谱可为两头尖的鉴别和质量评价提供参考。
Objective: To establish two sharp HPLC fingerprints. Methods: HPLC method was used. The chromatographic column was Phemomenex Gemini C18 with a mobile phase of 0.1% phosphoric acid-acetonitrile (gradient elution) at a flow rate of 1 ml / min with a detection wavelength of 206 nm and a column temperature of 30 ° C. The injection volume was 20 μl. Thirteen batches of two sharp samples were analyzed with the reference of sarcaxin A. Similarity analysis was carried out by using “similarity evaluation system of chromatographic fingerprints of traditional Chinese medicines”, and the samples were analyzed by SPSS 13.0. Results: There are 11 common peaks in 13 batches of two sharp samples, the similarity is> 0.90. Proved that the two sharp fingerprints and control fingerprints have good consistency. The origin of the 13 batches of medicinal herbs in the areas of Jilin Huadian, Jilin Jiaohe, Jilin Tigang, Jilin Zuojia, Heilongjiang Shangzhi, Jilin Shulan, Jilin Tonghua and Jilin Fusong are No. 1, Harbin, Heilongjiang, Yabuli, Heilongjiang, Qingyuan, Heilongjiang Slope, Shandong Jinan for the second category. Conclusion: The fingerprinted fingerprints can provide a reference for the identification and quality evaluation of two sharp points.