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本实验旨在研究熊果酸对正常人外周血淋巴细胞Th1型细胞因子的影响,并对机制作初步探讨。采用半定量RT-PCR和夹心法ELISA分别检测不同浓度熊果酸对PHA和PMA活化的外周血淋巴细胞的Th1型细胞因子(IFN-γ和IL-2)mRNA的表达及分泌的影响。结果显示Th1型细胞因子mRNA和蛋白的表达水平均随熊果酸浓度的增加而下降(P<0.05),表明熊果酸可能对IFN-γ和IL-2在转录和(或)转录后水平发挥抑制作用,有可能用于自身免疫性疾病和超敏反应性疾病的防治。而熊果酸和地塞米松分子结构相似,可能通过类似机制下调糖皮质激素受体发挥作用。
The purpose of this experiment was to study the effect of ursolic acid on Th1 cytokines in peripheral blood lymphocytes of normal people and to discuss the mechanism of the production. The effects of different concentrations of ursolic acid on the expression and secretion of Th1 cytokines (IFN-γ and IL-2) mRNA in peripheral blood lymphocytes activated by PHA and PMA were detected by semi-quantitative RT-PCR and sandwich ELISA. The results showed that the expression level of Th1 cytokine mRNA and protein decreased with the increase of ursolic acid concentration (P<0.05), indicating that ursolic acid may be transcriptionally and/or post-transcriptionally active against IFN-γ and IL-2. It exerts inhibitory effects and may be used for the prevention and treatment of autoimmune diseases and hypersensitivity diseases. The similar molecular structure of ursolic acid and dexamethasone may play a role in down-regulating the glucocorticoid receptor through a similar mechanism.