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采用小苍兰五个品种的花器作组织培养,均获得愈伤组织和试管苗。将活性炭不同浓度(0.0％、0.2％、0.5％、0.8％),加入含2,4-D0.1毫克/升+BA0.1毫克/升的MS培养基中,可使小苍兰愈伤组织的分化率从25％提高到70.6％以上。在试验范围内,随着活性炭浓度提高,所分化的苗叶色渐趋青绿,清秀;但苗长得过高,不便移植。用矮壮素加入上述培养基中可使苗矮壮。试验结果表明,以附加矮壮素5毫升/升的达到秆粗0.3厘米的试管苗,其矮化百分率最高,其次为3毫升/升的。试验结果还表明,花器诱导的再生植株可以有两种途径:先芽后根和先根后芽。在供试各品种中,以鲜黄增殖速度最快,大白、桔红次之,粉红、土蓝再次之。
Five species of freesia flowers for tissue culture, were callus and test tube seedlings. The different concentrations of activated carbon (0.0%, 0.2%, 0.5%, 0.8%), adding 2,4-D0.1 mg / L + BA0.1 mg / liter of MS medium, can make freesia callus The rate of tissue differentiation increased from 25% to over 70.6%. Within the experimental range, with the increase of activated carbon concentration, the differentiated seedling leaves gradually turned green and delicate, but the seedlings were too long for transplantation. Add Chlormequat to the medium above to make the shoots vigorous. The results showed that, with additional chlormequat 5 ml / l reach the stalk 0.3 cm thick tube seedlings, the highest dwarfing percentage, followed by 3 ml / l. The experimental results also showed that there are two ways for flower-induced regeneration of plants: first after the posterior roots and after the first root bud. In the test varieties, the fastest growth of fresh yellow, white, orange second, pink, blue again.