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本文报道朝鲜出血热病原因子(KHF)在体外人源细胞中首次传代成功。这种人源细胞取自一名肺癌患者的肺泡上皮细胞。命名为A—549,所用的初始感染材料是几只黑线姬鼠的肺组织混合悬液(10%),这些黑线姬鼠曾接种感染过第4代(KHF)76—118毒株的黑线姬鼠肺组织的混悬液,在接种后第21天处死。将得到的初始感染材料10~(-1)稀释液加入含有10%小牛血清E—199培养液,接种在用各种原代和传代鸟类及哺乳类动物组织制备的单层细胞(置于12毫米条状盖玻片)上。盖玻片上的单层细胞用5%小牛血清E—199培养液维持,间隔3～5天换液
This paper reports the first passage of KHF in human cells in vitro. This human-derived cell is taken from the alveolar epithelial cells of a lung cancer patient. Named A-549, the initial infectious material used was a mixture of lung tissue suspensions (10%) of several Apodemus agrarius, who had been infected with strains of infected fourth generation (KHF) strain 76-118 The suspension of Apodemus agrarius lung tissues was sacrificed on the 21st day after inoculation. The resulting initial dilutions of the infectious material 10-1 were added to a culture medium containing 10% fetal calf serum E-199 and inoculated into monolayer cells prepared from various primary and passage bird and mammalian tissues On 12 mm strip coverslips). Cover cells on a single layer of cells with 5% fetal calf serum E-199 culture maintained at intervals of 3 to 5 days replacement fluid