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【目的】评估不同类型煤炭生物降解转化为甲烷的潜力,研究原位煤层的微生物群落结构特征。【方法】分别在原位模拟、补加烃降解产甲烷菌系和补加碳源下厌氧培养煤样,利用气相色谱监测甲烷产生趋势,及高通量测序技术研究原位煤层的细菌和古菌群落。【结果】10个样品中有3个高成熟度煤样可以被厌氧降解转化为甲烷。通过生物强化和添加外源底物可以促进HF煤样的产甲烷潜力。其中SL煤样中的古菌类群主要是氢营养型产甲烷菌Methanoculleus和乙酸营养型产甲烷菌Methanosaeta为主,细菌类群主要属于Firmicutes(54.4%)、Proteobacteria(30.9%)、未培养微生物(10.8%)、Caldiserica(1.5%)及Thermotogae(1.3%)。【结论】不同成熟度煤样降解产气潜力不同,在部分原位煤层中可能存在参与烃降解与甲烷产生的功能菌。
【Objective】 To evaluate the potential of different types of biodegradation of coal to methane and to study the microbial community structure characteristics of coal seam in situ. 【Method】 The coal samples were cultured anaerobically in the in situ simulation, supplemented with hydrocarbon-degrading methanogenic bacteria and supplemented with carbon source. The methane production trend was monitored by gas chromatography and the high-throughput sequencing technology was used to study the bacteria and Archaeal community. 【Result】 Three samples with high maturity were converted to methane by anaerobic degradation. The methane-producing potential of HF samples can be enhanced by bioenhancing and adding exogenous substrates. Among them, the archaebacteria of SL coal samples are mainly Methanoculleus, a hydrogen-producing methanogen, and Methanosaeta, an acetic acid-producing methanogen. Bacteria mainly belong to Firmicutes (54.4%), Proteobacteria (30.9%), uncultured microorganisms 10.8%), Caldiserica (1.5%) and Thermotogae (1.3%). 【Conclusion】 Different mature coal samples have different potentials for degradation and gas production, and some functional bacteria may be involved in hydrocarbon degradation and methane production in some coal seams.