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以凉粉草带茎节的茎段为外植体,采用L9(34)正交设计,对影响凉粉草组培快繁过程的3个因素(6-BA、ZT、NAA)的3个浓度水平进行优化试验,试验结果采用SPSS13.0软件统计分析,并针对凉粉草在组培快繁过程中极易发生玻璃化等问题进行研究。结果表明:凉粉草的最适初代诱导培养基为MS+6-BA0.5 mg/L+NAA0.1 mg/L,无玻璃化;最适继代增殖培养基为MS+6-BA0.5 mg/L+ZT0.5 mg/L+NAA 0.02 mg/L+PVA 1000 mg/L,无玻璃化,增殖系数达9.1,芽壮;最适生根培养基为1/2MS+NAA0.1 mg/L+KT0.01 mg/L。1000 mg/L PVA为有效防治凉粉草组培快繁过程中玻璃苗发生的最佳浓度。采用这一技术,理论上1株试管苗一年可以生产凉粉草种苗约为9万株。
Using the stem segments of stem segments of Grifola frondosa as explants, the three concentrations (6-BA, ZT, NAA) of three factors affecting the process of tissue culture and rapid propagation of Glitter were determined by L9 (34) orthogonal design The optimization test was carried out. The test results were analyzed by SPSS 13.0 software and the problems of vitrification and so on were easily occurred in the process of tissue culture rapid propagation of jelly grass. The results showed that the optimal initial induction medium for MS was 6 mg / L MS + 6-BA + NAA 0.1 mg / L, with no vitrification. The best medium for subculture was MS + 6-BA 0.5 NAA 0.02 mg / L + PVA 1000 mg / L, without vitrification, the multiplication coefficient reached 9.1 and shoot vigor. The optimum medium for rooting was 1/2 MS + NAA 0.1 mg / L + KT0.01 mg / L. 1000 mg / L PVA is the optimal concentration of glass seedlings in the process of tissue culture and propagation of effective control of jelly grass. Using this technique, theoretically one test-tube plantlet can produce about 90,000 plants per year of glitter grass.