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以花生品种花育33为试材,根据cDNA文库中已知的果糖-1,6-二磷酸醛缩酶(fructose-1,6-bisphosphate aldolase,FBA)基因全长序列设计引物,通过RT-PCR克隆到该基因,命名为AhFBA1。AhFBA1全长为1489 bp,开放阅读框为1200 bp,编码400个氨基酸。预测该基因编码的蛋白含有Glycolytic保守结构域,可能定位于叶绿体中。蛋白序列比对和进化树分析表明,花生与大豆(Glycine max)、苜蓿(Medicago truncatula)、鹰嘴豆(Cicer arietinum)和菜豆(Phaseolus vulgaris)等豆科植物中的FBA序列相似性最高,亲缘关系最近。荧光定量PCR结果显示,在高盐和干旱胁迫下,AhFBA1在花生叶和根中的表达均受明显诱导,说明该基因可能参与花生对高盐和干旱胁迫的适应性调控;AhFBA1在花生根和叶中均受ABA的明显诱导,说明该基因对花生非生物胁迫的调控可能是依赖ABA的。
Based on the full-length sequence of fructose-1,6-bisphosphate aldolase (FBA) from the cDNA library of Huayu 33, which is a peanut variety, PCR cloned into the gene, named AhFBA1. AhFBA1 has a total length of 1489 bp and an open reading frame of 1200 bp, encoding 400 amino acids. It is predicted that the protein encoded by this gene contains a conserved Glycolytic domain, probably located in the chloroplast. Protein sequence alignment and phylogenetic tree analysis showed that peanut had the highest FBA sequence similarity with legumes such as Glycine max, Medicago truncatula, Cicer arietinum and Phaseolus vulgaris, Recent relationship. Fluorescence quantitative PCR results showed that under high salt and drought stress, AhFBA1 expression in the peanut leaves and roots were significantly induced, indicating that the gene may be involved in peanut adaptation to high salt and drought stress; AhFBA1 in the peanut root and The leaves were significantly induced by ABA, indicating that the gene of peanut abiotic stress regulation may be dependent on ABA.