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目的建立当归地上部分中绿原酸与金丝桃苷的含量测定方法。方法采用高效液相色谱法,色谱柱为GRACE C18(250mm×4.6mm,5μm);流动相为乙腈(A)-0.5%甲酸水溶液(B)梯度洗脱;流速1.0ml/min;检测波长为325nm(绿原酸)和355nm(金丝桃苷)。结果绿原酸和金丝桃苷的线性范围分别为0.29～5.8μg/μL(R2=0.9996),0.1～2.0μg/μL(R2=0.9999);平均加样回收率分别为为97.34%(RSD=4.77%),101.70%(RSD=3.07%)。结论该方法准确可靠、简便可行,可用于当归地上部分药材的质量控制。
Objective To establish a method for the determination of chlorogenic acid and hyperoside in aerial parts of Angelica sinensis. Methods The chromatographic column was GRACE C18 (250 mm × 4.6 mm, 5 μm). The mobile phase consisted of a gradient of acetonitrile (A) - 0.5% formic acid in water (B). The flow rate was 1.0 ml / 325 nm (chlorogenic acid) and 355 nm (hyperoside). Results The linear ranges of chlorogenic acid and hyperoside were 0.29 ~ 5.8μg / μL (R2 = 0.9996) and 0.1 ~ 2.0μg / μL (R2 = 0.9999) .The average recoveries were 97.34% = 4.77%), 101.70% (RSD = 3.07%). Conclusion The method is accurate and reliable, simple and feasible, and can be used for quality control of aerial parts of Angelica sinensis.