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目的探讨凋亡抑制基因Livin在喉癌组织中的表达及其与喉癌临床病理的相关性。方法采用免疫组织化学染色方法检测了50例喉癌组织,45例癌旁组织,10例喉乳头状瘤及16例声带息肉中Livin蛋白的表达情况。RT-PCR方法检测41喉癌组织,41例癌旁组织和11声带息肉组织中Livin mRNA的表达情况。结果 RT-PCR检测结果显示Livin mRNA在喉癌组织中相对表达量明显高于癌旁组织及声带息肉组织(P<0.01)。免疫组织化学检测结果显示Livin蛋白在喉癌组织中的阳性表达率明显高于癌旁组织、喉乳头状瘤及声带息肉(P<0.05)。Livin蛋白表达与肿瘤的病理分级有关(P<0.05),而与患者年龄、肿瘤部位、临床分期、淋巴结转移无关(P>0.05)。结论 Livin在喉癌组织中异常高表达,提示在喉癌的发生、发展中可能起重要作用。
Objective To investigate the expression of apoptosis-suppressing gene Livin in laryngeal carcinoma and its correlation with clinicopathological features of laryngeal carcinoma. Methods Immunohistochemical staining was used to detect the expression of Livin protein in 50 cases of laryngeal carcinoma, 45 cases of paracancerous tissues, 10 cases of laryngeal papilloma and 16 cases of vocal cord polyp. The expression of Livin mRNA in 41 laryngeal carcinoma tissues, 41 adjacent tissues and 11 vocal cord polyp tissues were detected by RT-PCR. Results The results of RT-PCR showed that the relative expression of Livin mRNA in laryngeal carcinoma tissues was significantly higher than that in paracancerous tissues and vocal cord polyps (P <0.01). The results of immunohistochemistry showed that the positive expression rate of Livin protein in laryngeal carcinoma was significantly higher than that in paracancerous tissues, laryngeal papilloma and vocal cord polyps (P <0.05). The expression of Livin was related to the pathological grade (P <0.05), but not to the age, tumor location, clinical stage and lymph node metastasis (P> 0.05). Conclusions Livin is highly expressed in laryngeal carcinoma, suggesting that Livin may play an important role in the occurrence and development of laryngeal carcinoma.