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目的 通过雌二醇 (E2 )、三苯氧胺 (TAM )作用于乳癌及子宫内膜细胞 ,研究这两种因素对雌激素受体 (ER)阳性细胞的增殖作用 ,探讨乳癌TAM耐受的产生与细胞来源的关系。方法1× 1 0 - 6 mol/LTAM、1× 1 0 - 8mol/LE2 作用于乳癌及子宫内膜细胞 ,利用3H TdR示踪细胞增殖动力学。结果 TAM对子宫内膜细胞增殖 (6 .32 % )的抑制作用与对照组 (6 .40 % )比较差异无显著性 (P <0 .0 1 ) ,TAM对乳癌细胞增殖 (45 .84% )的抑制作用与对照组 (52 .72 % )比较差异有显著性 (P <0 .0 1 ) ,但其对TAM耐受的乳癌细胞增殖 (9.64 % )与对照组 (6 .32 % )比较有显著刺激作用 ;E2 对 3种细胞增殖的刺激作用与对照组比较差异都有显著性。E2 与TAM联合作用于子宫内膜及乳癌细胞与单独使用E2 时相比 ,表现为抑制细胞增殖的作用 ;但它们联合作用于TAM耐受乳癌细胞时表现出比E2 更大的刺激细胞增殖的作用。结论 E2 对 3种来源细胞都表现为刺激增殖的作用 ,但TAM抑制乳癌细胞增殖的作用则依赖E2 的浓度水平而不同
OBJECTIVE: To investigate the effects of estradiol (E2) and tamoxifen (TAM) on breast cancer and endometrial cells and to study the effects of these two factors on the proliferation of estrogen receptor (ER) positive cells, Source of the relationship. Methods 1 × 10 ~ 6 mol / L TAM and 1 × 10 ~ 8 mol / L E 2 were used in breast cancer and endometrial cells. 3H - TdR was used to observe the cell proliferation kinetics. Results The inhibitory effect of TAM on endometrial cell proliferation (6.32%) was not significantly different from that of control group (6.40%) (P <0.01) ) Was significantly lower than that of the control group (52.72%) (P <0.01), but the proliferation of TAM-resistant breast cancer cells (9.64%) was significantly lower than that of the control group (6.32% Compared with control group, the stimulating effect of E2 on three kinds of cell proliferation was significant. E2 and TAM combined with endometrial and breast cancer cells showed inhibitory effects on cell proliferation compared with E2 alone. However, when combined with TAM, TAM-resistant breast cancer cells showed a greater stimulation of cell proliferation than E2 effect. Conclusion E2 exerts a stimulatory effect on proliferation of all three kinds of cells. However, the effect of TAM on the proliferation of breast cancer cells depends on the concentration of E2