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目的:研究痛啡肽(nociceptin)对大鼠离体气管/支气管的胆碱能神经兴奋所致收缩的抑制作用.方法:记录电场刺激引起胆碱能神经兴奋所致的标本收缩张力,了解nociceptin的作用.结果:Nociceptin0.001-0.1μmol/L可抑制标本的胆碱能收缩,其IC_(50)(95％的可信限)分别是0.06(0.04-0.08)μmol/L和0.07(0.05-0.1)μmol/L.在气管和支气管上,nociceptin 0.01μmol/L的抑制率分别是:(58±32)％和(60±26)％;预用纳洛酮0.1μmol/L后,nociceptin的抑制率为:(60±19)％和(54±20)％(P>0.05).Nociceptin 0.01μmol/L不影响外源性乙酰胆碱引起的气道标本收缩.κ阿片受体激动剂U-50488H 0.01—1 μmol/L不影响电场刺激引起的大鼠气道胆碱能收缩.结论:痛啡肽抑制电刺激引起的大鼠气道胆碱能收缩反应,且不受纳洛酮影响.
OBJECTIVE: To study the inhibitory effect of nociceptin on contraction induced by excitatory cholinergic activity in isolated trachea / bronchus of rats.Methods: The contraction of nociceptin (50%) (95% confidence interval) were 0.06 (0.04-0.08) μmol / L and 0.07 (0.05), respectively.Results: Nociceptin0.001-0.1μmol / L inhibited the cholinergic contraction of the samples, -0.1) μmol / L. In the trachea and bronchus, the inhibitory rates of nociceptin 0.01 μmol / L were (58 ± 32)% and (60 ± 26)%, respectively. After naloxone 0.1 μmol / (60 ± 19)% and (54 ± 20)%, respectively (P> 0.05) .Nociceptin 0.01μmol / L did not affect the contraction of airway samples induced by exogenous acetylcholine.K-opioid receptor agonist U- 50488H 0.01-1 μmol / L did not affect the cholinergic contraction induced by electric field stimulation in rats.Conclusion: Painkil inhibits the electrical cholinergic contraction induced by electrical stimulation in rats, and is not affected by naloxone.