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[Objective] Earlier identification of pregnant and non-pregnant animals post mating can greatly improve reproductive efficiency and pregnancy rate and decrease the interval between services.The experiment was conducted to develop a rapid and simple early pregnancy diagnostic method in sheep.[Method] The early pregnancy diagnosis was conducted with serum acid titration in 1 317 female sheep(Duolang sheep,Cele sheep and Karakul sheep) after 16-30 d post artificial insemination.[Result] After the serum acid titration,1 128 pregnant ewes and 189 non-pregnant ewes were detected in the three sheep breeds.Compared with the actual pregnancy detection,the coincidence rate of pregnancy was 89.88%(1 013/1 128);the coincidence rate of non-pregnancy was 96.83%(183/189);and the total coincidence rate was 90.81%(1 196/1 317) .Chi-square test analysis showed that no significant difference(P < 0.05) was found between the three sheep breeds or between any two breeds.[Conclusion] This serum acid titration is a simple,convenient and highly accurate method for early pregnancy diagnosis in sheep,which shows highly practical and popularizing value.
[Objective] Earlier identification of pregnant and non-pregnant animals post mating can greatly improve reproductive efficiency and pregnancy rate and decrease the interval between services. The experiment was conducted to develop a rapid and simple early pregnancy diagnostic method in sheep. [Method] The early pregnancy diagnosis was conducted with serum acid titration in 1 317 female sheep (Duolang sheep, Cele sheep and Karakul sheep) after 16-30 d post artificial insemination. [Result] After the serum acid titration, 1 128 pregnant ewes and 189 non- pregnant ewes were detected in the three sheep breeds. Compared with the actual pregnancy detection, the coincidence rate of pregnancy was 89.88% (1 013/1 128); the coincidence rate of non-pregnancy was 96.83% (183/189); and The total coincidence rate was 90.81% (1 196/1 317). Chi-square test analysis showed that no significant difference (P <0.05) was found between the three sheep breeds or between any two breeds. [Conclusion] This serum acid titratio n is a simple, convenient and highly accurate method for early pregnancy diagnosis in sheep, which shows highly practical and popularizing value.