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目的 了解２３个型的肺炎球菌荚膜多糖的稳定性。方法 分别用Ｓｅｐｈａｒｏｓｅ２Ｂ或４Ｂ凝胶柱测定 相对分子质量及其分布（Ｋｄ）。结果 用蓝色葡聚糖２０００和氧化钠作标准测定外水体积Ｖｏ（１５２．５６或１５４．０４ｍｉｎ）和 内水体积Ｖｔ（４３２．２３或４４４．０７ｍｉｎ），均有良好的分离效果。于２～８℃保存的所有型别的多糖均符合规程要求，除１、 ２、４、８、１２Ｆ、１４、１８Ｃ和２３Ｆ型多糖因基础相对分子质量较大有轻微降解外，其他均未发现有明显降解；于室温保存的 多糖除１、２、８、１２Ｆ、１４和１８Ｃ型有轻微降解外，其他型多糖降解程度均较大；于３７℃保存的多糖除１、２、８、１２Ｆ和１４ 型有轻微降解外，其他型则有更大程度的降解。结论 高温不利于多糖的保存。２３个型别的液态多糖于２～８℃保 存１年均稳定。
Objective To understand the stability of 23 pneumococcal capsular polysaccharides. Methods The relative molecular mass and its distribution (Kd) were determined by Sepharose 2B or 4B gel column respectively. Results The external water volume Vo (152.56 or 154.04 min) and the internal water volume Vt (432.23 or 444.07 min) were determined with blue dextran 2000 and sodium oxide as standard, which showed good separation effect. All types of polysaccharides preserved at 2-8 ° C met the regulatory requirements except that 1, 2, 4, 8, 12F, 14, 18C and 23F type polysaccharides slightly degraded due to the larger relative molecular weight The polysaccharides stored at room temperature except 1, 2, 8, 12F, 14 and 18C slightly degraded, and all the other polysaccharides degraded to a great extent. 12F and 14 slightly degraded, other types have a greater degree of degradation. Conclusion High temperature is not conducive to the preservation of polysaccharides. 23 types of liquid polysaccharides stored at 2-8 ℃ for 1 year were stable.