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The dating of fluid inclusions of quartz yields an Ar-Ar isochrone age of 320.4±6 Ma. Three types of fluid inclusions have been identified with the homogenization temperature ranging from 157℃ to 362℃. The homogenization temperature consists of two groups. The first group varies from 157℃ to 166℃, and the second from 232℃ to 362℃.Their chemical composition is dominated by Na+-Ca2+-Mg2+ and Cl-. The relative concentration of ions is characteristic by Na+＞Ca2+＞K+＞Mg2+ and C1-＞SO42-＞ F-. The δD and δ180 values indicate that the ore-forming fluid originates from mixing of multi-source water. The Sarkobu gold deposit has experienced two mineralization stages: gold was enriched during the volcanic-exhalative-sedimentary process in the early stage, while the gold deposit was finally formed under compression-shearing during the orogenic period.