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用ELISA法和PCR法对 5 2例非甲非乙型急性肝炎和 2 0例输血后肝炎患者进行检测 ,结果用ELISA法检出抗—HCV阳性 4 0例 ,阳性率 5 5 6% ;PCR法检出HCVRNA4 9例 ,阳性率 68 10 %。 4 0例抗—HCV阳性患者均检出HCVRNA ,9例抗—HCV阴性患者检出HCVRNA。结果显示PCR法检出率明显高于ELISA法。认为用特异性强、敏感性高的方法检测肝炎病毒 ,可最大限度地降低漏检 ,是防止肝炎病毒通过输血等途径传播的最有效的措施。
A total of 52 non-A, non-A and 20 post-transfusion hepatitis patients were detected by ELISA and PCR. Results The positive anti-HCV antibodies were detected by ELISA. The positive rate was 55.6% Ninety-nine cases of HCV RNA were detected with a positive rate of 68.10%. HCV RNA was detected in 40 anti-HCV positive patients and HCV RNA was detected in 9 anti-HCV negative patients. The results showed that PCR detection rate was significantly higher than the ELISA method. It is considered that the detection of hepatitis virus by a specific and sensitive method can minimize the missed inspection and is the most effective measure to prevent the hepatitis virus from being transmitted by blood transfusion.