论文部分内容阅读
目的探讨健脾消癥方对人肝癌细胞增殖、凋亡的影响及可能的机制。方法 MTT法观察健脾消癥方对HepG2、SMMC-7721增殖的影响;Annexin V/PI双染色法观察健脾消癥方对HepG2凋亡的影响;实时荧光定量PCR法检测健脾消癥方对HepG2的凋亡相关基因Bax、Bcl-2、Bak的mRNA表达的影响;Western blot检测健脾消癥方对HepG2细胞凋亡相关蛋白Bcl-2、Bax表达的影响。结果健脾消癥方对HepG2、SMMC-7721均有良好的增殖抑制作用,对HepG2的抑制作用尤为显著;Annexin V/PI双染显示,健脾消癥方作用HepG2 48h后出现明显的早期凋亡;实时荧光定量PCR证实健脾消癥方作用HepG2 48 h后,Bak、Bax基因mRNA表达上升,Bcl-2基因mRNA表达下降,Bcl-2/Bax比例下降;Western blot证实健脾消癥方作用HepG2 48 h后,Bax蛋白表达上升,Bcl-2蛋白表达下降。结论健脾消癥方对HepG2有良好的抑制增殖、诱导凋亡的作用;其作用机制可能与健脾消癥方下调Bcl-2、上调Bak Bax基因mRNA表达,下调Bcl-2蛋白,上调Bax蛋白有关。
Objective To investigate the effects of Jianpi Xiaoji prescription on the proliferation and apoptosis of human hepatoma cells and its possible mechanism. Methods MTT method was used to observe the effect of Jianpixiaoji prescription on the proliferation of HepG2 and SMMC-7721 cells. The effect of Jianpixiaozheng prescription on HepG2 apoptosis was observed by Annexin V / PI double staining method. The effect of Jianpi Xiaozheng prescription On HepG2 apoptosis-related genes Bax, Bcl-2, Bak mRNA expression; Western blot detection of Jianpi Xiaozheng Fang HepG2 apoptosis-related proteins Bcl-2, Bax expression. Results Jianpixiaojifang had good proliferation inhibitory effect on HepG2 and SMMC-7721, especially on HepG2. Annexin V / PI double staining showed that HepG2 had obvious early apoptosis after HepG2 treatment Real-time PCR confirmed that the expression of Bak, Bax mRNA and Bcl-2 mRNA decreased and the ratio of Bcl-2 / Bax decreased after 48 hours HepG2 treatment. After 48 hours of HepG2 treatment, the expression of Bax increased and the expression of Bcl-2 decreased. Conclusion Jianpixiaozheng can inhibit HepG2 proliferation and induce apoptosis effectively. The mechanism may be related to down-regulating Bcl-2, up-regulating Bak Bax mRNA expression, down-regulating Bcl-2 protein and up-regulating Bax Protein related.