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为了获得一种高效、洁净的姜酚分离方法,选用乙醇-水体系为柱层析方法流动相,固定相为D4020大孔吸附树脂,从超临界二氧化碳提取的姜油树脂中分离姜酚。用紫外分光光度法对各段洗脱液进行跟踪检测,确定含姜酚的洗出段。用高效液相色谱法(high performance liquid chromatography,HPLC)和气相色谱质谱联用法(gas chromatography-mass spectrometer,GC-MS)对含姜酚的洗脱液进行检测。研究结果表明,在保证86.11%收率的条件下,姜酚的峰面积百分数为71.98%;经二次柱层析纯化后,姜酚的峰色谱面积百分数可达96.27%,收率为76.75%。该方法在分离纯度和收率上都获得了好的效果。
In order to obtain an efficient and clean method for the separation of gingerol, ethanol-water system was selected as the mobile phase of column chromatography. The stationary phase was D4020 macroporous resin. Gingerol was separated from ginger oleoresin extracted with supercritical carbon dioxide. UV spectrophotometry for each section of the eluent tracking test to determine the washout section with gingerol. Ginger-containing eluent was detected by high performance liquid chromatography (HPLC) and gas chromatography-mass spectrometer (GC-MS). The results showed that under the condition of 86.11% yield, the peak area of gingerol was 71.98%. After purification by quadratic column chromatography, the peak area of gingerol was 96.27% and the yield was 76.75% . The method has good effect on separation purity and yield.