论文部分内容阅读
大部分固相酶标免疫试验是以抗体或抗原在塑料表面的吸附为基础。但是,在一个简单的物理吸附过程中,被吸附的抗原或抗体的量是很不充足的,使方法的敏感度受到了限制,而且,吸附的蛋白质在免疫试验的过程中会脱落,从而引起重复性不良。用吸附制备的固相抗体无法重复使用也是一个未曾解决的问题。在传统的酶联免疫吸附试验(ELISA)中,高达40～68%的吸附抗体或抗原将在测定过程中脱落。如不用吸附手段,改用化学键,使抗原或抗体和载体相连就可以克服这一缺点。作者使用直径3.2～6.4mm的聚环己烷-已二酰二胺尼龙珠为载体,在3.5 M盐酸中处理24小时,经蒸馏水冲洗后再用浓醋酐处理1分钟,使尼龙部分水解,在其表面产生游离的氨基和羧基。这些带化学功能基的尼龙珠可用4%碳二亚胺(CMC)或8%戊二醛活化。将活
Most solid phase enzyme immunoassays are based on the adsorption of antibodies or antigens on plastic surfaces. However, in a simple physical adsorption process, the amount of antigen or antibody being adsorbed is insufficient, the sensitivity of the method is limited, and the adsorbed protein may fall off during the course of the immunoassay, resulting in Poor repeatability. Solid-phase antibody prepared by adsorption can not be used repeatedly is also an unsolved problem. In traditional ELISA assays up to 40-68% of adsorbed antibodies or antigens will fall off during the assay. Without the use of adsorption means, the use of chemical bonds, the antigen or antibody and carrier connected to overcome this shortcoming. The authors used polycyclohexane-adipamide beads with a diameter of 3.2-6.4mm as carriers and treated them in 3.5 M hydrochloric acid for 24 hours. After rinsing with distilled water and then with concentrated acetic anhydride for 1 minute, the nylon was partially hydrolyzed, On its surface free amino and carboxyl groups are produced. These chemically functionalized nylon beads can be activated with 4% carbodiimide (CMC) or 8% glutaraldehyde. Will live