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目的:了解广东地区地方株HPV18L1基因结构特点,为HPV感染的检测和基因工程疫苗的研制提供实验基础。方法:采用PCR技术从广东地区宫颈癌组织中扩增HPV18L1基因,克隆入毕赤酵母表达载体pPICZαB,测序并对HPV18L1基因进行序列分析。结果:广东地区分离株与德国标准株在核苷酸序列上有4处不同,其中3处由于核苷酸的改变,其编码的氨基酸也发生相应变化,与标准株的同源性为99.8%。与中国西安地区分离株比较有两处突变点相同。结论:广东地区HPV18L1分离株与德国标准株、中国其他地区分离株之间均存在差异。
OBJECTIVE: To understand the structural characteristics of HPV18 L1 gene in endemic strains in Guangdong Province, and to provide an experimental basis for the detection of HPV infection and the development of genetic engineering vaccines. Methods: The HPV18 L1 gene was amplified from cervical cancer tissue of Guangdong province by PCR and cloned into the expression vector pPICZαB of Pichia pastoris. The HPV18 L1 gene was sequenced and sequenced. Results: There were 4 different nucleotide sequences in Guangdong isolates and German standard strains. Among them, 3 of them had corresponding amino acid changes due to nucleotide changes. The homologies with the standard strains were 99.8% . Compared with the isolates from Xi’an, China, there are two identical mutation points. Conclusion: There are differences between HPV18 L1 isolates from Guangdong and other isolates from Germany.