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目的:研究血管内皮生长因子(VEGF)与血小板反应蛋白(TSP-1)在膀胱移行细胞癌中的表达及其与膀胱癌血管生成的关系。方法:应用免疫组织化学技术检测48例原发性膀胱移行细胞癌和10例正常膀胱组织中VEGF和TSP-1的表达,测定肿瘤组织的微血管密度(MVD),分析VEGF、TSP-1和MVD三者之间关系及它们与膀胱癌病理分级和临床分期之间的关系。结果:VEGF、TSP-1和MVD肿瘤的临床分级和临床分期密切相关。膀胱癌中．VEGF表达高的,MVD值也高;而TSP-1表达强的,MVD值低(P<0．05)。中度或高度表达VEGF而TSP-1阴性的肿瘤,MVD均值较高;VEGF低表达而TSP-1阳性的肿瘤,MVD均值较低。中度表达VEGF的膀胱癌中,TSP-1低者,MVD值较高;TSP-1表达强者, MVD值较低(P<0．05)。VEGF、TSP-1表达与术后复发有关(P<0．05)。结论:VEGF、TSP-1表达与膀胱移行细胞癌的发生发展有关,是膀胱癌血管形成的重要原因。膀胱癌组织中VEGF、TSP-1的测定可作为判断预后的有用指标。从多方面着手抗血管生成治疗膀胱癌也可能更加有效。
Objective: To investigate the expression of vascular endothelial growth factor (VEGF) and thrombospondin (TSP-1) in bladder transitional cell carcinoma and its relationship with angiogenesis in bladder cancer. Methods: The expression of VEGF and TSP-1 in 48 cases of primary bladder transitional cell carcinoma and 10 cases of normal bladder tissues were detected by immunohistochemistry. The microvessel density (MVD) in tumor tissue was measured. The expressions of VEGF, TSP-1 and MVD The relationship between the three and their relationship with the pathological grade and clinical stage of bladder cancer. Results: The clinical grade and clinical stage of VEGF, TSP-1 and MVD tumors were closely related. Bladder cancer. The expression of VEGF was high and the MVD was also high. However, the expression of TSP-1 was strong and MVD was low (P <0.05). MVDs were moderate or highly expressed in TSP-1-negative tumors, with higher mean MVD; tumors with low VEGF expression and TSP-1-positive tumors had lower mean MVD. In bladder cancer with moderate expression of VEGF, TSP-1 had higher MVD, TSP-1 had higher expression and MVD was lower (P <0.05). The expressions of VEGF and TSP-1 were correlated with recurrence (P <0.05). Conclusion: The expression of VEGF and TSP-1 is related to the occurrence and development of transitional cell carcinoma of the bladder, which is an important reason for the angiogenesis of bladder cancer. The determination of VEGF and TSP-1 in bladder cancer can be used as a useful indicator to judge the prognosis. In many ways, anti-angiogenic treatment of bladder cancer may also be more effective.