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目的 观察急性肺损伤小鼠T辅助淋巴细胞 (TH) 1/TH2的功能变化 ,探讨其在急性肺损损伤发生中的作用。方法 小鼠腹腔注射脂多糖复制急性肺损伤模型。ELISA测定脾细胞培养上清液中干扰素 (1FN) -γ、白细胞介素 (IL) 4浓度 ,RT PCR测定细胞因子mRNA表达。伊文思蓝荧光法测定肺血管蛋白渗出量。结果 与正常对照组比较 ,急性肺损伤组动物肺组织中伊文思蓝渗出量、肺湿重 /干重比均明显升高。在刀豆素A刺激的脾细胞上清液中 ,急性肺损伤组IFN γ浓度显著下降 (P<0 0 1) ,IL 4明显升高 (P <0 0 1)。RT PCR显示急性肺损伤组动物脾细胞IFN γmRNA表达明显下降。结论 急性肺损伤时 ,THl向TH2漂移 ,以抗炎症反应为主 ,调控抗炎反应是急性肺损伤治疗的关键。
Objective To investigate the function of T helper lymphocyte (TH) 1 / TH2 in mice with acute lung injury and to explore its role in the pathogenesis of acute lung injury. Methods Mice were injected intraperitoneally with lipopolysaccharide to establish a model of acute lung injury. The concentration of interferon (IFN-γ) and interleukin (IL) 4 in spleen cell culture supernatant was measured by ELISA. The mRNA expression of cytokines was measured by RT PCR. Evans Blue Fluorescence Assay for Pulmonary Vascular Protein Exudation. Results Compared with the normal control group, the excretion of Evans blue and the ratio of wet weight to dry weight of lung in acute lung injury group were significantly increased. In the concanavalin-stimulated splenocyte supernatant, the concentration of IFN-γ in acute lung injury group was significantly decreased (P <0.01), and IL 4 was significantly increased (P <0.01). RT-PCR showed that the expression of IFN-γmRNA in splenocytes of acute lung injury group was significantly decreased. Conclusions In acute lung injury, the TH1 shift to TH2, which is mainly anti-inflammatory and the anti-inflammatory response is the key to the treatment of acute lung injury.