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目的 探讨转化生长因子 β1(TGF β1)对ⅩⅧ型胶原在大鼠肾小球系膜细胞中表达的影响 ,以及两者在大鼠抗Thy 1肾小球肾炎中的相互关系。方法 采用RT PCR、Westernblot法观察外源性TGF β1对大鼠肾小球系膜细胞中ⅩⅧ型胶原表达的影响 ;制备大鼠抗Thy 1肾小球肾炎模型 ,应用免疫组织化学ABC方法分别观察TGF β1和ⅩⅧ型胶原在大鼠肾组织中的表达 ,并对其染色强度作图像定量分析。结果 外源性TGF β1作用后 ,大鼠肾小球系膜细胞中ⅩⅧ型胶原的表达升高 ,并显示出一定的时间与浓度依赖性。大鼠抗Thy 1肾小球肾炎中TGF β1的表达在 1、3和 5d仅有微量表达 ,第 7天开始其表达明显且随时间增强 ;ⅩⅧ型胶原和Ⅳ型胶原的表达趋势都与TGF β1吻合 ,ⅩⅧ型胶原的表达与TGF β1的表达间具有显著的相关性。结论 ⅩⅧ型胶原的表达与TGF β1的作用相关 ,可能参与了肾小球肾炎的发生发展过程 ,并且可能在肾小球纤维化中起一定的作用
Objective To investigate the effect of transforming growth factor-β1 (TGF-β1) on the expression of collagen type ⅩⅧ in rat glomerular mesangial cells and the relationship between them in rat anti-Thy 1 glomerulonephritis. Methods The effects of exogenous TGFβ1 on the expression of collagen type ⅩⅧ in rat mesangial cells were observed by RT PCR and Western blotting. The rat model of anti-Thy 1 glomerulonephritis was prepared and observed by immunohistochemical ABC method TGF β1 and ⅩⅧ collagen expression in rat kidney tissue, and its staining intensity for image quantitative analysis. Results The expression of collagen type ⅩⅧ in rat glomerular mesangial cells increased with exogenous TGF-β1 and showed a certain time-dependent and concentration-dependent manner. The expression of TGF-β1 in rat anti-Thy-1 glomerulonephritis was only slightly expressed on 1, 3 and 5 days, and the expression of TGF-β1 was obvious on the 7th day and increased with time. The expressions of type ⅩⅧ collagen and type Ⅳ collagen showed the same tendency as TGF β1 anastomosis, the expression of type ⅩⅧ collagen and TGF β1 have a significant correlation between expression. Conclusion The expression of type ⅩⅧ collagen is related to the effect of TGF-β1, which may be involved in the development of glomerulonephritis and may play a role in glomerular fibrosis