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目的观察阿司匹林对小鼠S180肉瘤生长的影响,并探讨其可能的作用机制。方法昆明种小鼠40只,接种S180肉瘤细胞,随机分为荷瘤对照组、5-氟尿嘧啶(5-FU)组、阿司匹林高(250 mg/kg)、低剂量(50 mg/kg)组,描绘肿瘤体积生长曲线并计算抑瘤率;HE染色观察各组肿瘤组织病理变化;免疫组化检测各组肿瘤组织微血管密度(MVD)、淋巴管密度(LVD)、血管内皮生长因子-A(VEGF-A)、血管内皮生长因子-C(VEGF-C)的变化;Western blot检测各组肿瘤组织VEGF-A、VEGF-C的表达。结果阿司匹林高、低剂量组抑瘤率分别为45.4%和22.2%(P<0.05),与荷瘤对照组相比,5-FU组与阿司匹林高、低剂量组VEGF-A和VEGF-C的表达明显下调,LVD、MVD降低(P<0.05),阿司匹林的作用呈剂量依赖性。结论阿司匹林能抑制小鼠S180肉瘤的生长,其作用机制可能与减少VEGF-A和VEGF-C产生从而抑制肿瘤血管和淋巴管生成有关。
Objective To observe the effect of aspirin on the growth of S180 sarcoma in mice and to explore its possible mechanism. Methods Forty Kunming mice were inoculated with S180 sarcoma cells and randomly divided into 5 groups: tumor-bearing control group, 5-fluorouracil group, aspirin high dose (250 mg / kg), low dose The tumor volume growth curve was plotted and the tumor inhibition rate was calculated. The pathological changes of the tumor tissue were observed by HE staining. The expression of MVD, LVD, VEGF -A) and VEGF-C. Western blot was used to detect the expression of VEGF-A and VEGF-C in each group. Results The inhibitory rates of aspirin in high and low dose groups were 45.4% and 22.2%, respectively (P <0.05). Compared with tumor-bearing control group, the inhibitory rates of VEGF-A and VEGF-C in high and low dose aspirin groups The expression was down-regulated, LVD and MVD were decreased (P <0.05), and the effect of aspirin was dose-dependent. Conclusions Aspirin can inhibit the growth of S180 sarcoma in mice. Its mechanism may be related to the decrease of VEGF-A and VEGF-C production and the inhibition of tumor blood vessels and lymphangiogenesis.