目的:建立用高效液相色谱法测定芒果止咳片中马来酸氯苯那敏含量的方法。方法:用Diamonsil C18(250 mm×4．6 mm,5μm)色谱柱,流动相为乙腈-0．1％十二烷基硫酸钠溶液-磷酸(60:40:0．1),流速1．0 ml·min-1,检测波长为263 nm。结果:对照品线性范围为0．04-2．0μg,r=1．000 0;平均回收率为99．0％,RSD=1．4％(n=5)。结论:用本法控制芒果止咳片片中的马来酸氟苯钠敏的含量,操作简便,结果准确。 Objective: To establish a method for the determination of chlorphenamine maleate in mango cough tablets by high performance liquid chromatography. Methods: Diamonsil C18 (250 mm × 4.6 mm, 5 μm) was used as the mobile phase. The mobile phase was acetonitrile - 0.1% sodium dodecyl sulfate solution - phosphoric acid (60:40: 0.1). 0 ml · min-1, detection wavelength of 263 nm. Results: The linear range of the reference substance was 0.04-2.0 μg, r = 1.000 0; the average recovery was 99.0% and the RSD was 1.4% (n = 5). Conclusion: This method can be used to control the content of flurbiprofen maleate in mango cough tablet easily and the result is accurate.