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水稻穗部性状与产量直接相关,定位克隆穗部性状相关QTL对探究穗部性状分子机制及分子植物育种具有重要意义。以粳稻TY319和籼稻保持系8B的F3群体为作图群体,对穗长、一次枝梗数、二次枝梗数、每穗总粒数和着粒密度进QTL定位与上位性分析。采用完备区间作图,共检测出14个穗部相关性状QTL,分别位于第1、第2、第7、第8、第9、第10染色体,LOD值介于2.56~4.96,表型变异贡献率介于6.90%~31.99%。控制二次枝梗数的qSB-1、及控制着粒密度的qSD-1和qSD-2未见报道,可能是新的QTL,上位性分析检测到69个互作。
The panicle traits of rice are directly related to the yield, and the QTLs for mapping panicle traits are of great significance in exploring the molecular mechanisms of panicle traits and molecular plant breeding. The F3 populations of japonica rice TY319 and indica rice maintainer line 8B were used as the mapping population. QTL mapping and epistasis analysis were performed on the spike length, primary branch number, secondary branch number, total grain number and grain density. Using complete interval mapping, 14 QTLs for panicle-related traits were detected in the first, second, seventh, eighth, ninth and tenth chromosomes with LOD values ranging from 2.56 to 4.96 and phenotypic variation contributions Rates ranged from 6.90% to 31.99%. QSB-1 controlling the number of secondary branches and qSD-1 and qSD-2 controlling grain densities were not reported, which may be new QTLs. 69 interactions were detected by epistasis analysis.