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文章通过培养的方法分析了小麦根际微生物的群落结构以及对常用畜禽用抗生素的敏感性,研究结果表明,小麦根际土壤微生物总数为5.251×108cfu/g,其中细菌占绝对优势,占总菌数的99.04%,放线菌占0.09%,真菌占0.06%。从中分离出10株优势细菌,经过分子生物学鉴定均与芽孢杆菌属(Bacillus)亲缘关系较近。这10株优势菌对土霉素和四环素的敏感性研究结果表明,大部分菌株对土霉素的敏感性高于四环素,不同的菌株对同一种抗生素的敏感性差异较大。10株菌对土霉素的敏感浓度在10~100μg/L之间,对四环素的敏感浓度在100~500μg/L之间。
The article analyzed the community structure of wheat rhizosphere microorganisms and their sensitivity to common livestock and poultry antibiotics through culturing methods. The results showed that the total number of soil microbial rhizosphere soil was 5.251 × 108cfu / g, of which bacteria accounted for the absolute dominant, accounting for 99.04% of bacteria, actinomycetes 0.09%, 0.06% of fungi. 10 strains of dominant bacteria were isolated from them. All of them were closely related to Bacillus by molecular biology. The sensitivity of ten dominant bacteria to oxytetracycline and tetracycline showed that the sensitivity of most strains to oxytetracycline was higher than that of tetracycline, and the sensitivity of different strains to the same antibiotic was quite different. Ten strains of oxytetracycline sensitive concentrations of 10 ~ 100μg / L between the sensitive concentration of tetracycline in the 100 ~ 500μg / L between.