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[目的]探讨微波及γ射线单独或联合照射对人神经胶质瘤细胞(SHG44细胞)热休克蛋白70(HSP70)表达的影响。[方法]SHG44细胞按是否接受γ射线照射(5Gy)分为单独微波组(M组)和射线微波联合组(IM组);两组又均以接受微波强度不同(0、2、4、6mW/cm2)分为4个亚组,即M0、M2、M4、M6亚组和IM0、IM2、IM4、IM6亚组。用四甲基偶氮唑盐(MTT)法测定细胞存活率,用western blot和RT-PCR方法检测HSP70的表达。[结果]与M0组相比,M4、M6、IM6组的细胞存活率均明显下降(P<0.05或P<0.01),尤以IM6组下降为甚;且IM6组的细胞存活率也低于单独γ射线照射组(IM0组),P<0.01。与M0组相比,各剂量M组的HSP70表达差异无统计学意义;经γ射线照射后,即各IM组的HSP70表达较M0组均明显下降(P<0.01);而与IM0组相比,各IM组的HSP70表达差异无统计学意义(P>0.05)。[结论]本实验条件下,微波和γ射线单独或联合辐射均能降低细胞存活率;γ射线照射能抑制HSP70的表达,但微波不能引起HSP70表达的明显变化。
[Objective] To investigate the effects of microwave and gamma irradiation on the expression of heat shock protein 70 (HSP70) in human glioma cells (SHG44 cells) alone or in combination. [Methods] SHG44 cells were divided into microwave group (M group) and radiation microwave group (IM group) according to whether they received γ-ray irradiation (5Gy) / cm2) is divided into 4 subgroups, namely M0, M2, M4, M6 subgroups and IM0, IM2, IM4, IM6 subgroups. The cell viability was determined by MTT assay, and the expression of HSP70 was detected by western blot and RT-PCR. [Results] Compared with M0 group, the cell viability in M4, M6 and IM6 groups decreased significantly (P <0.05 or P <0.01), especially in IM6 group, and the cell survival rate in IM6 group was also lower than Gamma irradiation alone group (IM0 group), P <0.01. Compared with the M0 group, there was no significant difference in the expression of HSP70 among the groups of M at each dose; after the γ-ray irradiation, the HSP70 expression in each IM group was significantly lower than that in the M0 group (P <0.01) There was no significant difference in the expression of HSP70 in each IM group (P> 0.05). [Conclusion] Under the experimental conditions, microwave and γ-ray radiation alone or in combination can reduce the cell survival rate; γ-ray irradiation can inhibit the expression of HSP70, but microwave can not cause significant changes in the expression of HSP70.