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目的 :探讨酸性神经肽 (bovineacidicneuropeptide 1BANP 1)对小鼠脑内氨基酸类递质 (谷氨酸和γ 氨基丁酸 )及cGMP的影响。方法 :将小鼠随机分为 6组 (每组 15只 ) ,其中 5组按 15mg/kg腹腔注射BANP 1,将小鼠在注射后 0 5h ,1h ,2h ,3h ,4h处死并取脑匀浆 ,另一组为注射生理盐水对照组 ,在注射盐水后 1h处死并取脑匀浆 ,用 75 %冰乙醇抽提氨基酸类物质及cGMP并沉淀蛋白质。应用滤纸电泳法对小鼠大脑谷氨酸 (glutamicacid ,Glu)和γ 氨基丁酸 (γ aminobutyricacid ,GABA)进行测定 ,应用放射免疫法对小鼠大脑环磷酸鸟苷 (cyclicmonophos phateguanosine ,cGMP)进行测定。结果 :腹腔注射BANP 1后 ,各项指标在不同时间的结果分别与对照组比较 :Glu浓度在 1h、2h时明显高于对照组 (P <0 0 1) ,0 5h、3h、4h时差异无显著性 (P >0 0 5 )。GABA浓度在 2h、3h时显著高于对照组 (P <0 0 1) ,0 5h、1h、4h时差异无显著性 (P >0 0 1)。cGMP浓度有一定升高 ,1h 2h时显著升高 (P <0 0 1) ,其余各时间组差异无显著性 (P >0 0 5 )。结论 :BANP 1急性用药后在一定时间可升高脑内Glu、GABA及cGMP水平
Objective: To investigate the effects of bovine acid methyl peptide 1BANP 1 on amino acid neurotransmitters (glutamate and γ-aminobutyric acid) and cGMP in mice brain. Methods: The mice were randomly divided into 6 groups (15 in each group). 5 of them were intraperitoneally injected with BANP 1 at 15mg / kg, and the mice were sacrificed at 5h, 1h, 2h, 3h and 4h after injection and brain homogenized The other group was injection of normal saline control group, which was sacrificed at 1h after injection of saline and brain homogenate. Amino acids and cGMP were extracted with 75% ethanol and precipitated protein. The contents of glutamic acid (Glu) and γ-aminobutyric acid (GABA) in mouse brain were determined by filter paper electrophoresis. The levels of cyclic monophosphatidylinositol (cGMP) in mice brain were determined by radioimmunoassay . Results: After intraperitoneal injection of BANP 1, the results at different time points were compared with those of the control group. The Glu concentration at 1 hour and 2 hours was significantly higher than that of the control group (P <0.01), and at 0-5 hours, 3 hours and 4 hours No significant (P> 0.05). GABA concentration was significantly higher at 2h and 3h than that of control group (P <0.01). There was no significant difference at 0h, 1h and 4h (P> 0.01). The concentration of cGMP increased to some extent at 1h and 2h (P <0.01), while there was no significant difference between the other groups (P> 0.05). Conclusion: After acute administration of BANP 1, Glu, GABA and cGMP levels can be increased in a certain period of time