新鲜巨核细胞是否有c-sis基因表达尚未阐明,作者发现一例10个月的巨核细胞白血病伴Down氏综合征的病孩的新鲜巨核细胞和建立的细胞株上有c-sis表达。作者用快速印迹法,此白血病的各临床阶段的巨核细胞中均有c-sis表达,而淋巴细胞和其他白血病细胞均无c-sis表达。复发时,外周血及骨髓细胞中的c-sis mRNA水平较治疗前高出5倍以上。另一例5个月的急性巨核细胞性白血病的细胞也有c-sis mRNA表达。将患者的原始细胞培养建立巨核细胞株(CMK)。这种巨核细胞与原来的原始细胞的染色体同样表现为der(17)t(11∶17)。在 Whether fresh megakaryocytes have c-sis gene expression has not been elucidated. The authors found c-sis expression on fresh megakaryocytes and established cell lines from a 10-month-old patient with Down’s syndrome. The authors used fast-blotting, in which all stages of leukemia have megakaryocytes in which c-sis is expressed, while none of lymphocytes and other leukemic cells express c-sis. At recurrence, c-sis mRNA levels in peripheral blood and bone marrow cells were more than 5-fold higher than before treatment. Another case of acute megakaryocytic leukemia at 5 months also had c-sis mRNA expression. The patient’s primary cells were cultured to establish a megakaryocyte cell line (CMK). This megakaryocyte also behaves like the original primordial chromosome der (17) t (11:17). in