【摘 要】
：
To explore the effects of traditional herbal medicine Ganoderma tsugae(G.tsugae) on immunomodulatory and antitumor activities,the crude polysaccharides ofG.tsug
【机 构】
：
College of Life Science,Department of Cosmetology,Department of Osteology,College of Chemistry
【基金项目】
：
Supported by the National Natural Science Foundation of China
论文部分内容阅读
To explore the effects of traditional herbal medicine Ganoderma tsugae(G.tsugae) on immunomodulatory and antitumor activities,the crude polysaccharides ofG.tsugae were purified by filtration,diethylaminoethyl(DEAE)sepharose-fast flow chromatography and sephadex G-100 size-exclusion chromatography.Two main fractions,protein-containing glycans CSSLP-I and CSSLP-2,were obtained via the gradient elution.The protein content,molecular weight,and monosaccharide composition of the two fractions were analyzed.Furthermore,the influence of the protein-containing glycans from G.tsugae on the activation of human acute monocytic leukemia cell line(THP-1 ) and their antitumor activities to the human hepatocellular liver carcinoma cell(HepG-2) in vitro were evaluated.The results indicate that CSSLP-I and CSSLP-2 could increase the pinocytic activity of THP-1 cells and induce THP-1 cells to produce the cytokines of TNFa and IL-2,significantly.CSSLP-1 and CSSLP-2 also played an inhibiting effect on the cancer cell(NepG-2).Moreover,the anti-proliferation activity of CSSLP-1 and CSSLP-2 increased with the participation of TNFa and 1L-2 or other antitumor factors induced from THP-1 cclls by G.tsugae protein-containing glycan fractions.
其他文献
运用石蜡切片技术和扫描电镜技术对稀有鲫（Gobiocypris rarus）鳃结构进行观察，并采用浓度为2 mg／L的 CdCl2水溶液对稀有鲫进行急性毒理实验。结果显示：稀有鲫具4对鳃，由鳃耙、鳃弓
Convincing evidence for a past interaction between the two rich clusters A399 and A401 was recently found in the X-ray imaging observations. We examine the stru
采用逆转录聚合酶链式反应（RT－PCR）方法，从哲罗鲑（Hucho taimen）肝脏的总 RNA 中扩增出胰岛素样生长因子－I（IGF－I）的 cDNA 开放阅读框（Open reading frame， ORF）序列，运用软件对其进行生物
根据Gen Bank中公布的鲑鱼甲病毒(salmonid alphavirus,SAV)SAV 1、SAV 2和SAV3三个基因型中E1基因,选择高保守序列702 bp(436-1137)合成基因,命名为SAV E1,将其克隆到原核表
为了分离鉴定qRL4,以超级稻协优9308衍生重组自交系与轮回亲本中恢9308(R9308)回交多代的高代回交群体为材料,利用琼脂无土栽培技术,开展水稻根长QTL qRL4的分离鉴定研究,最
This report documents the treatment of a giant aneurysm of the internal carotid artery bifurcation with a fistula to the cavernous sinus,which appeared followin
Primary systemic amyloidosis is a relatively rare disease,caused when abnormal extracellular deposition of fibrillary protein builds up in a variety of target o
为在起落架系统的半实物仿真中模拟起落架控制系统所需的所有航电、飞控信号,利用ARINC429板卡,在LabWindows/CVI平台上设计实现了液压起落架信号模拟系统.通过与仿真目标机
基于时间相关单光子计数(TCSPC)技术原理,研究了一套面向早期乳腺癌检测的时域荧光层析成像系统,系统采用非接触式空间光扫描测量方式,相对于传统的光纤接触式测量方法,可增
采用宏观形貌、化学成分、能谱等分析方法对某废水管道法兰螺栓服役过程中断裂的现象进行了分析。结果表明,螺栓断裂的原因为氯离子作用下的应力腐蚀开裂,铬和镍的含量过低会