论文部分内容阅读
目的 :探讨ISH在诊断HBV -GN的意义及不同病理形态的HBV -DNA的分布部位 ,数量及分布状况与病理类型的关系。方法 :分别采用免疫荧光法 ,聚合酶链式反应 (PCR) ,原位杂交 (ISH)法检测肾组织中HBV标志物的存在状况。结果 :在 4 2例患儿中 ,通过检测患儿肾组织中HBV -Ag ,诊断 2 1例为HBV -GN ;PCR法检测的HBV标志物阳性率最高 ,为 6 0 .0 % ,ISH法次之 ,为 4 5 .2 4 % ;HBV -DNA在不同病理形态中 ,不同部位的阳性细胞率各不相同 ,在膜性肾病中 ,上皮细胞的阳性率明显高于其它病理类型 (P <0 .0 5 )。而膜增殖性肾炎主要分布在内皮与系膜区细胞中 ;在我们的研究中发现 :单纯应用免疫荧光法明确HBV -GN的诊断 ,对膜性HBV -GN的漏诊率较高 ;而对膜增生性HBV -GN的误诊率太高。结论 :应用原位杂交法检测肾组织中HBV -DNA的存在状况对HBV -GM的确诊有重要意义 ;应用原位杂交法检测肾组织中HBV -DNA的分布部分对明确病理类型有重要的诊断价值 ;应用原位杂交法检测肾组织中HBV -DNA的分布部位及存在状况对探讨HBV -GN的发病机制有重要意义。
Objective: To investigate the significance of ISH in the diagnosis of HBV-GN and the distribution of HBV-DNA in different pathological forms of the location, number and distribution of the relationship with the pathological type. Methods: The presence of HBV markers in renal tissues was detected by immunofluorescence, polymerase chain reaction (PCR) and in situ hybridization (ISH) respectively. Results: Twenty-two cases were diagnosed as HBV-GN by detecting HBV-Ag in renal tissues of 42 children. The positive rate of HBV markers detected by PCR was 60.0%, ISH method Followed by 45.24%; HBV-DNA in different pathological forms, different parts of the positive rate of different cells in membranous nephropathy, the positive rate of epithelial cells was significantly higher than other pathological types (P < 0 .0 5). The proliferative glomerulonephritis was mainly distributed in the cells of endothelial and mesangial cells. In our study, it was found that the diagnosis of HBV-GN by simple immunofluorescence was higher than that of membranous HBV-GN. However, Misdiagnosis rate of proliferative HBV-GN is too high. Conclusion: The detection of HBV-DNA in renal tissue by in situ hybridization is of great significance for the diagnosis of HBV-GM. The in situ hybridization assay of HBV-DNA in renal tissue is of important diagnostic value for definite pathological types Value; using in situ hybridization to detect the distribution of HBV-DNA in renal tissue and the existence of the location of the pathogenesis of HBV-GN is of great significance.