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用~(51)Cr标记的蛙红细胞作为放射性生物微球,测定了正常成年麻醉家兔视网膜-脉络膜、睫状体-虹膜及巩膜的血流量.此技术简便易行,用途较广,可用于眼科部分缺血性眼病的实验研究.放射性微球技术系Rudolph及Heymann于1967年首创用于研究血流的分布,心输出量及器官局部血流量.目前该技术在国外实验室中广泛采用,公认其为对血管无创伤而合乎生理的一种研究方法其原理为:用放射性同位素标记的人造微球,其直径比毛细血管内径稍大,将其注入左心室或左心房后,会随血液循环到全身各脏器,第一次通过脏器时即被嵌顿于毛细血管中,按流过该脏器的血流量的比例,分布到各脏器和组织,根据测得的各脏器放射性,可求出任一脏器血流量及占心输出量的百分比.国外所用放射性微球主要为美国3M公司生产的碳化微球,此外尚有瑞典生产的萄聚糖微球,均系专利产品,价格昂贵.1973年Baehlei等曾用~(99M)TC标记牛蛙红细胞注入狗的左心室或肾动脉,研究肾皮质血流的分布,首次提出了放散性生物微球的概念.1981年我国学者王景贤等首先应用~(51)Cr标记的蛙红细胞作为一种放射性生物微球,成功地测定了家兔部分组织的局部血流量,本实验采用~(51)Cr标记的蛙红细胞作为放射性微球测定了家兔部分眼组织的血流量,国内、外尚未见报道.
The blood flow of the retina - choroid, ciliary body - iris and sclera of normal adult anesthetized rabbits was determined using ~ (51) Cr - labeled frog erythrocytes as radioactive biological microspheres.This technique was simple, Experimental study of ophthalmic partial ischemic eye disease Radiation microsphere technology Rudolph and Heymann pioneered the study of the distribution of blood flow, cardiac output and organ blood flow in 1967. At present, the technology is widely used in foreign laboratories, Recognized as a vascular non-invasive and physiologically a research method The principle is: with radioisotope labeled artificial microspheres, the diameter slightly larger than the capillary diameter, its injection into the left ventricle or left atrium, with the blood Circulation to the various organs of the body, the first time that organism was implanted in capillaries, according to the proportion of blood flow through the organs, distributed to various organs and tissues, according to the measured organ Radioactivity, can be calculated for any organ blood flow and the percentage of cardiac output.Radio microspheres used abroad mainly for the United States 3M Company produced carbonized microspheres, in addition there are still produced in Sweden dextran microspheres, are patented product ,price Expensive.In 1973, Baehlei et al. (2009) have used ~ (99M) TC labeled bullfrog erythrocytes into the left ventricle or renal artery of dogs to study the distribution of renal cortical blood flow, for the first time put forward the concept of diffuse biological microspheres. Firstly, ~ (51) Cr-labeled frog erythrocytes were used as a kind of radioactive biological microsphere to successfully measure the local blood flow in some tissues of rabbits. The ~ (51) Cr-labeled frog erythrocytes were used as the radioactive microspheres Part of rabbit eye tissue blood flow, at home and abroad have not been reported.