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为了解流化床生物滤器内部细菌群落组成及其净水机制,通过高通量测序方法,研究了不同时期滤器中表层和底层滤料的细菌群落结构,分析了滤器不同床层的营养盐变化情况及水处理性能.结果表明,滤器的硝化作用主要发生于床层下部,表层对其的贡献率不显著.稳定工况下,流化床生物滤器对NH_4~+-N、TN、BOD_5和SS的去除率达到(68.3±2.24)%、(49.54±3.56)%、(60.35±4.98)%和(45.21±2.11)%,对氨氮的去除负荷达到(343.28±75.5)g·(m~3·d)~(-1),其硝化性能优于常规生物滤器.试验共筛选31个门,490个细菌属,其生物多样性显著高于常规生物滤器.自清洗装置的启停对滤器中不同区域载体表面细菌的多样性没有影响,对各样品的优势菌群略有影响.在滤器稳定运行时,表层区域的优势细菌基本维持不变,主要包括厌氧绳菌科、黄杆菌科、红杆菌科、硝化螺菌属、暖绳菌科.而底层区域的优势细菌随着时间的推移有所变化,主要包括硝化螺菌属、微丝菌属、Muricauda、Defluviimonas、红杆菌科.
In order to understand the bacterial community composition and its mechanism of water purification in the fluidized bed biofilter, the bacterial community structure of the surface and bottom filters in different periods of filter was studied by high-throughput sequencing method. The changes of nutrients in different beds of the filter The results showed that the nitrification of the filter mainly occurred in the lower part of the bed, and the contribution of the surface layer was not significant.Under steady conditions, the effect of the fluidized bed biofilter on the NH_4 ~ + -N, TN, BOD_5 and The removal efficiency of SS was (68.3 ± 2.24)%, (49.54 ± 3.56)%, (60.35 ± 4.98)% and (45.21 ± 2.11)%, respectively. The removal of ammonia reached 343.28 ± 75.5 g · (m ~ · D) ~ (-1), its nitrification performance is better than that of conventional biofilter.Test screening a total of 31 gates, 490 bacteria genus, its biological diversity was significantly higher than the conventional biofilter.Starting and stopping of self-cleaning device in the filter The bacterial diversity on the surface of the carrier in different regions had no effect on the dominant microflora of each sample, and the dominant bacteria in the surface layer remained unchanged during the stable operation of the filter, including Anaerobe, Flavobacterium, Rhizoctoniaceae, Nitrospirae, and Cordyceps, while the advantages of the bottom area are fine Over time varies, including Nitrospira, actin genus, Muricauda, Defluviimonas, red bacilli Branch.