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目的探讨冷暴露对血清中谷胱甘肽(GSH)、超氧化物歧化酶(SOD)及丙二醛(MDA)的影响。方法将90只Wistar大鼠随机分为1个对照组,10个冷暴露组,观察大鼠冷暴露过程中及复温后血清中GSH、SOD和MDA含量的变化。结果GSH含量在冷暴露过程中升高,60min达峰值[(318.29±47.02)mg/L],复温后12h时最低[(184.63±35.97)mg/L],与对照组的(254.74±61.56)mg/L比较,差异均有显著性(P<0.01)。SOD在复温后4h时明显降低[(138.34±17.53)×103U/L],与对照组(160.17±12.32)×103U/L比较差异有显著性(P<0.01)。MDA含量在冷暴露过程中升高,60min为(22.04±5.51)μmol/L、80min为(23.29±3.45)μmol/L,在复温后第4h达到峰值[(65.76±11.61)μmol/L],与对照组的(8.69±4.76)μmol/L比较差异均有显著性(P<0.01)。结论冷暴露可以使抗氧化物含量发生变化。
Objective To investigate the effects of cold exposure on serum glutathione (GSH), superoxide dismutase (SOD) and malondialdehyde (MDA). Methods Ninety Wistar rats were randomly divided into a control group and 10 cold exposure groups. The changes of GSH, SOD and MDA in serum after cold exposure were observed. Results The content of GSH increased during cold exposure, reaching the peak at 60 min [(318.29 ± 47.02) mg / L] and the lowest at 12h after rewarming (184.63 ± 35.97 mg / L) compared with the control group (254.74 ± 61.56 ) mg / L, the difference was significant (P <0.01). SOD was significantly lower at 4h after rewarming [(138.34 ± 17.53) × 103U / L] compared with the control group (160.17 ± 12.32) × 103U / L (P <0.01). MDA content increased during cold exposure (22.04 ± 5.51 μmol / L for 60 min and (23.29 ± 3.45) μmol / L for 80 min and reached peak at 4 h after rewarming (65.76 ± 11.61 μmol / L] , Compared with the control group (8.69 ± 4.76) μmol / L, the difference was significant (P <0.01). Conclusion Cold exposure can change the content of antioxidants.