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目的观察不同月龄的自发性高血压大鼠(SHR)的肾脏血管紧张素转换酶2(ACE2)的mRNA和蛋白质表达水平,探讨ACE2与血压状态的内在联系。方法12周龄雄性自发性高血压大鼠(SHR)18只和12周龄Wistar-Kyo-to(WKY)大鼠18只。随机分为SHR组和WKY组,每组抽取各9只处死后与喂养12周后处死的24周龄大鼠一并进行ACE2的检测。采用实时定量RT-PCR法检测ACE2 mRNA的表达,免疫组织化学检测ACE2蛋白的表达。结果与同周龄WKY组比较,SHR组大鼠的血压显著增加(P均<0.001),ACE2 mRNA表达显著降低(P均<0.001);与12周龄SHR组比较,24周龄SHR的血压显著增加(P<0.01),ACE2 mRNA表达显著降低(P<0.01);与同周龄的WKY组比较,SHR组肾脏ACE2免疫染色表达显著减少。结论肾脏ACE2 mRNA和蛋白质的表达与血压升高相关。
Objective To observe the mRNA and protein expression of renal angiotensin converting enzyme 2 (ACE2) in spontaneously hypertensive rats (SHR) of different months and to explore the relationship between ACE2 and blood pressure status. Methods Eighteen male 12-week-old spontaneously hypertensive rats (SHR) and 18 Wistar-Kyo-to (WKY) rats aged 12 weeks were enrolled. Randomly divided into SHR group and WKY group, 9 rats in each group were sacrificed 12 weeks after feeding and 24 weeks of age rats were also ACE2 detection. The expression of ACE2 mRNA was detected by real-time quantitative RT-PCR, and the expression of ACE2 protein was detected by immunohistochemistry. Results Compared with the WKY group, the blood pressure of SHR group was significantly increased (P <0.001) and the expression of ACE2 mRNA was significantly decreased (P <0.001). Compared with the 12-week SHR group, the blood pressure (P <0.01), while the expression of ACE2 mRNA in SHR group was significantly lower than that in WKY group (P <0.01). Conclusion The expression of ACE2 mRNA and protein in kidney is related to the increase of blood pressure.