研究了乙酰胺的分光光度分析方法。该方法用溴水氧化酰胺 ,其氧化产物可把 I-氧化成 I2 ,I2 再和淀粉 -KI生成有色络合物来测定酰胺的含量 ,主要适用于伯酰胺的分析。方法在 5× 1 0 - 6 ～ 1× 1 0 - 4 g/ 2 5m L范围内符合比耳定律 ,表观摩尔吸光系数 ε5 76 nm=4 .5× 1 0 4L·mol- 1·cm- 1。该法灵敏、快速、重现性好。 The acetamide spectrophotometric method was studied. The method oxidizes the amide with bromine water, and the oxidation product can oxidize I-oxide to I2, then form colored complex with starch-KI to determine the content of amide, which is mainly suitable for the analysis of primary amide. The method conforms to the Beer ’s law in the range of 5 × 10 ~ 6 ~ 1 × 10 4 g / 2 5m L with an apparent molar absorptivity ε5 76 nm = 4.5 × 10 4 L · mol -1 · cm -1. 1. The method is sensitive, fast and reproducible.