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目的研究通心络胶囊对大鼠缺血再灌注损伤的保护作用及其作用机制。方法99只SD大鼠随机分为通心络治疗组、通心络预防组及未用药对照组,线栓法建立缺血2h再灌注损伤模型,再灌注后1h、1d和5d断头取脑。TTC测定脑梗死体积;干湿法计算脑含水量;电镜观察海马神经元超微结构;羟胺法检测SOD含量;TBA法检测MDA含量;比色法检测GSH-PX、钠-钾-ATP酶、NO含量;免疫荧光观察小胶质细胞活化情况。结果在通心络药物干预下,大鼠脑梗死侧和梗死对侧含水量减少,脑梗死体积减小,缺血性海马神经细胞坏死肿胀减轻,SOD、GSH-PX和钠-钾-ATP酶含量增加,MDA、NO含量降低(P<0.05),小胶质细胞的表达减少。结论通心络胶囊可能通过调节自由基、减轻钙内流和抑制炎症反应的作用机制减轻脑缺血再灌注损伤。
Objective To study the protective effect and mechanism of Tongxinluo Capsule on ischemia-reperfusion injury in rats. Methods 99 SD rats were randomly divided into Tongxinluo treatment group, Tongxinluo prevention group and non-drug control group. The model of ischemic 2h reperfusion injury was established by thread embolism method. The brains were decapitated at 1h, 1d and 5d after reperfusion. . The volume of cerebral infarction was measured by TTC; brain water content was calculated by dry-wet method; ultrastructure of hippocampal neurons was observed by electron microscope; SOD content was detected by hydroxylamine method; MDA content was detected by TBA method; GSH-PX, sodium-potassium-ATPase, and colorimetric method were measured by colorimetry. NO content; Immunofluorescence observation of microglia activation. RESULTS: Under the intervention of Tongxinluo drugs, the water content of cerebral infarction side and contralateral side of infarction decreased, the volume of cerebral infarction decreased, the necrosis and swelling of ischemic hippocampus decreased, SOD, GSH-PX and sodium-potassium-ATPase. The contents of MDA and NO decreased (P<0.05), and the expression of microglia decreased. Conclusion Tongxinluo capsule may reduce cerebral ischemia-reperfusion injury by regulating free radicals, reducing calcium influx and inhibiting the inflammatory response.