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目的:初步探讨白藜芦醇(resveratrol,RV)对小鼠氧惊厥潜伏期及大发作时间的影响及可能机制,以期寻找预防氧惊厥的新途径。方法:应用小鼠氧惊厥模型(纯氧5 ATA暴露),尾静脉注射不同剂量RV(1.5、5、15 mg/kg),采用行为学观察方法,测定氧惊厥潜伏期及大发作时间;并测定RV 5 mg/kg注射剂量下不同时间点(5 ATA氧暴露后5、15、30 min)脑组织丙二醛(maleic dialdehyde, MDA)含量。结果:与对照组相比,RV 5、15 mg/kg组氧惊厥潜伏期及大发作时间均明显延长(P<0.01),而RV 1.5 mg/kg组无显著差异。尾静脉注射RV 5 mg/kg后,RV 5、15、30 min各组顶叶皮质与海马MDA随氧暴露(5 ATA)时间的延长,呈现逐步升高的趋势;但与相应时间点对照组相比,RV 5、15 min组顶叶皮质和海马MDA含量均明显降低(P<0.01),RV 30 min组皮质和海马MDA含量无统计学差异。结论:RV可能通过其抗氧化特性延长氧惊厥潜伏期,从而保护脑组织。
OBJECTIVE: To investigate the effects of resveratrol (RV) on the latency and the duration of major episodes of oxygen convulsions in mice, and to explore possible mechanisms for the prevention of oxygen convulsions. METHODS: Mouse oxygen convulsion model (Oxygen-5 ATA exposure) was used. Different doses of RV (1.5, 5, 15 mg/kg) were injected through the tail vein. Behavioral observations were used to determine the latency of oxygen convulsion and the time of major attack. The content of malonic dialdehyde (MDA) in brain tissue at different time points (5, 15 and 30 min after 5 ATA oxygen exposure) at 5 mg/kg RV injection dose. RESULTS: Compared with the control group, the latency and the duration of major episodes of oxygen convulsion in the RV 5 and 15 mg/kg groups were significantly prolonged (P<0.01), while there was no significant difference in the RV 1.5 mg/kg group. After intravenous administration of RV 5 mg/kg, the MDA of the parietal cortex and hippocampus in RVs at 5, 15 and 30 min increased progressively with the prolonged exposure of oxygen (5 ATA); however, it was associated with the corresponding time points in the control group. In contrast, MDA content in parietal cortex and hippocampus of RV 5 and 15 min groups was significantly decreased (P<0.01). MDA content in cortex and hippocampus of RV 30 min group was not statistically different. CONCLUSIONS: RV may prolong the latency of oxygen convulsions by its antioxidant properties, thereby protecting brain tissue.