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目的观察从玉米麸皮中提取的低聚糖阿魏酸酯(corn feruloyl oligosaccharides,CFOs)体外对H2O2诱导损伤的PC12细胞的保护作用,探讨其抗氧化作用及机制。方法采用H2O2建立PC12细胞损伤模型,应用MTT法检测各组PC12细胞的增殖率;倒置显微镜下观察细胞形态的变化;流式细胞术测定细胞凋亡率;试剂盒检测PC12细胞内及培养液中LDH、SOD、MDA活力及含量。结果除25μmol/L CFOs组外,其余CFOs各组的细胞活力(A值)与H2O2组相比,差别均有统计学意义(P<0.05),且随着CFOs浓度的加大,细胞活力有逐渐增强的趋势。在800μmol/L时,细胞存活率达到最高值。CFOs的抗氧化作用强度与其浓度存在量效及时效双重依赖性关系。与H2O2组相比,CFOs低、中、高浓度组细胞形态均有所好转,随浓度升高,贴壁细胞数量明显增加,细胞突触逐渐增长并趋于正常。细胞凋亡率均低于H2O2组,且具有浓度依赖性,细胞内外MDA含量明显减少;细胞液中LDH活力明显减弱;细胞内SOD活力显著增强(P<0.01)。结论 CFOs对经H2O2诱导的PC12细胞具有保护作用,该作用的机制与提高PC12细胞的抗氧化能力相关。
Objective To observe the protective effect of oligosaccharides oligosaccharides (CFOs) extracted from corn bran on H2O2-induced injury of PC12 cells in vitro and to explore their anti-oxidation mechanism. Methods PC12 cell injury model was established by H2O2. The proliferation rate of PC12 cells in each group was detected by MTT method. The morphological changes of cells were observed under inverted microscope. The apoptosis rate of PC12 cells was detected by flow cytometry. LDH, SOD, MDA activity and content. Results Compared with H2O2 group, the cell viability (A value) of CFOs in all other groups except the 25μmol / L CFOs group were significantly different (P <0.05). With the increase of CFOs concentration, the cell viability Increasing trend. At 800μmol / L, cell survival reached the highest value. There was a dose-dependent and time-dependent double-dependent relationship between the intensity of antioxidant activity and the concentration of CFOs. Compared with H 2 O 2 group, CFOs cells in low, middle and high concentration groups all improved in morphology. With the increasing of concentration, the number of adherent cells increased obviously, and the synapses gradually increased and became normal. The apoptotic rate of HUVECs was lower than that of H 2 O 2 groups, and in a concentration-dependent manner. The contents of MDA in the cells were significantly decreased. The activity of LDH in the cells was significantly decreased. The activity of SOD in the cells was significantly increased (P <0.01). Conclusion CFOs have a protective effect on PC12 cells induced by H2O2, the mechanism of which is related to the anti-oxidation ability of PC12 cells.