目的:确定原肠胚前期胚胎对发育毒性药物敏感的时间点,并建立评价Cyc诱导胚胎发育异常机制的分子生物学指标。方法:大鼠孕d 3 ip Cyc 10,20,40 mg·kg~(-1),孕d 8 SDS-聚丙烯酰胺凝胶电泳定位大鼠胚胎蛋白。结果:Cyc 40 mg·kg~(-1)组70 kDa蛋白表达显著上调,14.4 kDa胚泡化特征性蛋白表达消失。结论:大鼠妊娠d 8为研究原肠胚前期胚胎发育毒性的较适时间点,孕d 8 70 kDa蛋白表达上调伴随14.4 kDa胚泡化特征性蛋白表达下调可作为该发育阶段胚胎毒性分子生物学评价指标。 OBJECTIVE: To determine the time point when preimplantation embryos are sensitive to developmental toxic drugs and to establish the molecular biological indexes to evaluate the mechanism of cyc induction of embryogenesis abnormalities. Methods: The rat embryo protein was localized by SDS-polyacrylamide gel electrophoresis on rat d 3 ip Cyc, 10, 20, 40 mg · kg -1. Results: The expression of 70 kDa protein in Cyc 40 mg · kg -1 group was significantly up-regulated and the expression of 14.4 kDa blastocyst-like protein disappeared. CONCLUSION: Pregnancy d 8 is the most suitable time point to study the virulence of preimplantation embryo development. Upregulation of d 8 70 kDa protein expression accompanied with the down-regulation of 14.4 kDa blastocyst expression may serve as embryonic virulence molecular biomarkers Learning evaluation index.