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目的:探讨多巴胺D3受体激动剂BP897对多发性抽动症模型小鼠靶器官的毒副作用,为临床安全用药提供动物实验依据。方法:将70只ICR雄性小鼠随机分为BP897低、中、高剂量组及正常对照组、模型组、恢复组、氟哌啶醇组,用亚氨基二丙腈[200 mg/(kg.d)]建立多发性抽动症小鼠模型,分别给予生理盐水、BP897(0.3 mg/kg、1.0 mg/kg、3.0 mg/kg)、氟哌啶醇(0.5 mg/kg)灌胃治疗,检测各组小鼠的血常规、尿生化、强直性木僵时间及重要脏器病理切片。结果:BP897各治疗组小鼠血常规、尿生化与模型组比较差异无统计学意义(P>0.05),肝脏、肾脏、脑组织无明显病理改变,强直性木僵时间短,提示锥体外系反应小,高剂量引起胃粘膜轻度炎性改变,但损伤是可逆的。结论:BP897对多发性抽动症模型小鼠毒副作用较小,是相对安全的多巴胺D3受体激动剂。
OBJECTIVE: To investigate the toxic side effects of dopamine D3 receptor agonist BP897 on target organ in mice with tic disorder and to provide animal experimental evidence for clinical safety. Methods: Seventy ICR male mice were randomly divided into BP897 low, medium and high dose groups and normal control group, model group, recovery group, haloperidol group, with iminodiacetic acid [200 mg / (kg. d)] to establish a mouse model of Tourette’s disease. The rats were given normal saline, BP897 (0.3 mg / kg, 1.0 mg / kg, 3.0 mg / kg) and haloperidol Each group of mice blood, urine biochemistry, tonic ankylosing stool and pathological sections of important organs. Results: There was no significant difference in blood routine and urinalysis between the BP897 treatment group and the model group (P> 0.05). There were no obvious pathological changes in the liver, kidney and brain tissues, Small reaction, high dose caused mild inflammatory changes in gastric mucosa, but the damage is reversible. Conclusion: BP897 is a relatively safe dopamine D3 receptor agonist with less toxic and side effects on mice with Tourette’s disease.