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本文报道根据Huggins等方法制备的、于-70℃～-75℃保存一年半的冷冻红细胞的实验研究和临床观察结果。该制品于输注后24小时的体内存活率为74.2％,酵解率为73.7％,红细胞悬浮液游离血红蛋白为11.2毫克％,残存甘油为0.638克％,红细胞形态基本完整,证明符合临床输注要求。经临床使用证明,对于抗出血性休克和纠正贫血等都可应用,且安全有效。
This article reports the experimental study and clinical observation of frozen red blood cells prepared according to the method of Huggins et al. At -70 ℃ ~ -75 ℃ for one and a half years. The in vivo survival rate of the product at 24 hours after infusion was 74.2%, the rate of fermentation was 73.7%, the free hemoglobin of erythrocyte suspension was 11.2 mg%, the residual glycerol was 0.638% and the morphology of erythrocytes was basically intact, which proved to be in line with clinical infusion Claim. Proved by clinical use, for hemorrhagic shock and correct anemia can be applied, and safe and effective.