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N.Strampelli是由意大利引入我国的小麦持久抗病性品种,对我国目前多数的条锈菌流行小种均有良好的抗性。为了明确其抗条锈病基因的遗传机制,利用小麦条锈病小种CYR30、CYR31、Su-4和Su-14对N.Strampelli与中国春杂交后代进行遗传分析,结果表明N.Strampelli对CYR30、CYR31的抗病性均由1对显性基因和1对隐性基因互补控制,对Su-14、Su-4的抗病性各由1对隐性基因控制,将其中控制Su-14抗病性的隐性基因暂时命名为YrNS-1。利用分离群体分析法(BSA)对接种Su-14的正交F2代群体进行SSR分子标记,在1BL上找到4个与YrNS-1紧密连锁的微卫星标记Xwmc719、Xgwm124、Xwmc44和Xcfa2147,遗传距离分别为3.2、4.6、5.7和10.3cM。与已知位于1BL染色体上的抗条锈基因比较分析表明,YrNS-1可能是1个新的抗条锈病基因。
N.Strampelli is a long-lasting wheat resistant variety introduced into Italy by Italy and has good resistance to most of the endemic races of stripe rust in our country. In order to clarify the genetic mechanism of stripe rust resistance genes, the genetic analysis of the wheat stripe rust resistant wheat CYR30, CYR31, Su-4 and Su-14 using N.Strampelli and Chinese spring hybrids showed that N.Strampelli had no significant effect on CYR30, CYR31 The disease resistance of Su-14 and Su-4 was controlled by one recessive gene, and the control of Su-14 resistance was controlled by one pair of dominant genes and one pair of recessive genes. The recessive gene was temporarily named YrNS-1. SSR markers were used to identify F2 populations of Su-14 inoculated with BSA. Four microsatellite markers Xwmc719, Xgwm124, Xwmc44 and Xcfa2147 closely linked to YrNS-1 were found on 1BL. The genetic distance Respectively 3.2, 4.6, 5.7 and 10.3 cM. The results showed that YrNS-1 might be a new stripe rust resistance gene, compared with the known stripe rust gene located on chromosome 1BL.